PRODUCT DESCRIPTION

Vegan Bifidobacterium bifidum HH-BB27 is a bacterial species of the genus Bifidobacterium. It is one of the most common probiotic bacteria that can be found in the body of mammals, including humans. Because no aminal-origin material is used in the production, this probiotic is suitable for vegetarians.

SPECIFICAITON

Strain name	Bifidobacterium bifidum	Strain number	HH-BB27
Potency	100bn cfu/g	Gene Identification	Yes
Deposit	CCTCC	Certification	ISO9001/ISO22000/FSSC22000/HACCP/HALAL/KOSHER/FDA
Packing	500g,1kg or 5kg per foil bag	Storage	-18℃ for long time, 2-8℃ for short time

TECHNICAL INDICATORS

Items		Specification
1) Live Bifidobacterium bifidum potency		≥ 1.0×1011 CFU/g
2) Water content		≤8.0%
4) Coliforms		≤10CFU/g
5) Moulds, Yeasts		≤10CFU/g
6) Pathogenic bacteria		Negative
7) Heavy metals	Lead (Pb)	≤1ppm
	Arsenic (As)	≤0.5ppm

RELEVANT RESEARCH

Strain Name	Identification	Deposit No.	Antibiotic Susceptibility	Acid and Bile Salt resistance	Stability	Quality System
B.bifidum HH-BB27	16S rRNA	-	Done	Done	Done	SC/ISO9001/ISO22000/FSSC22000/ HACCP/HALAL/ KOSHER/FDA

ACID TOLERANCE

Acid resistance of Bifidobacterium bifidum HH-BB27 powder was carried out at either pH=2.0 and pH=3.0 for up to 4 hours. The microbial enumeration of Bifidobacterium bifidum HH-BB27 should be performed no more frequently than at the initial(as control), 1 hour, 2 hour, and 4 hour time points. The Fig 1 illustrates that Bifidobacterium bifidum HH-BB27 can survive at low pH conditions, which means this strain has good acid resistance.

Fig 1. Acid resistance of B.bifidum HH-BB27

BILE SALT TOLERANCE

Bile salt resistance of Bifidobacterium bifidum HH-BB27 powder was carried out at either 0.2% and 0.3% bile salt for up to 4 hours. The microbial enumeration of Bifidobacterium bifidum HH-BB27 should be performed no more frequently than at the initial(as control), 1 hour, 2 hour, and 4 hour time points. The Fig 2 illustrates that Bifidobacterium bifidum HH-BB27 can survive at high concentration of bile salt, which means this strain has good bile salt resistance.

Fig 2. Bile salt resistance of B.bifidum HH-BB27

STORAGE STABILITY

Bifidobacterium bifidum HH-BB27 powder were sealed in aluminum foil bag, maintained at -18℃ for 24 months for long-term stability test. The microbial enumeration of Bifidobacterium bifidum HH-BB27 should be performed no more frequently than at the initial, 1, 2, 3, 6, 9, 12, 15, 18, 21, 24 month time points. According to Fig 3, the potency of Bifidobacterium bifidum HH-BB27 powder was 2.41×10¹¹ CFU/g. After a storage period of 24 months, the potency of the powder was 1.97×10¹¹CFU/g. It means that this strain can remain stable for long time if stored at low temperature.

Fig 3. Storage stability of B.bifidum HH-BB27

HEALTH BENEFITS

Activities against H.pylori

Helicobacter pylori infection occurs when H. pylori bacteria infect your stomach. This usually happens during childhood. Complications associated with H. pylori infection include ulcers, inflammation of the stomach lining, even stomach cancer. In experiments, we found that B.bifidum HH-BB27 can inhibit H.pylori growth and its adhension to intestinal mucus.

	Diam of H.pylori inhibition（mm）
Strain	H.pylori NCTC11637	H.pylori NCTC11638
B.bifidum HH-BB27	7.18±0.38	6.89±0.46

Table 1. Diam of H.pylori inhibition

	Inhibition of adhesion of H. pylori to intestinal mucus (%)
Strain	H.pylori NCTC11637	H.pylori NCTC11638
B.bifidum HH-BB27	8.33±0.41	7.19±0.28

Table 2. Inhibition of adhesion of H. pylori to intestinal mucus

APPLICATIONS

Infant formula, food supplements and dairy products

OTHER HEALTH BENEFITS

1. Fights against weight gain/Obesity

2. Improves and protects the health and appearance of skin

3. Alleviation of allergies

4. Alleviation of constipation

5. Combats the harmful effects of asthma

PACKING AND DELIVERY

COMPANY IMAGE

http://www.probioway.com/